Beskrivning
Eppendorf Mastercycler® X50
Advanced PCR Optimization in a One Step
Mastercycler X50 PCR thermocyclers have a 2D-gradient function that means you can simultaneously optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) – see the example of β-actin gene PCR optimization.
Higher denaturation temperatures can increase specificity while lower denaturation temperatures can increase yield, so with the MC X50 PCR cyclers, you can quickly determine the conditions for both optimal yield and specificity. This saves PCR optimization time, is a great benefit in low target copy number and GC-rich target applications, and is really useful in troubleshooting non-specific amplification issues.
Need rapid PCR results?
In the modern lab, fast is essential, not a luxury. PCR thermocyclers that give you quicker results throughout the day and a faster transition to optimal temperatures mean you can get through more work in a shorter time. The Mastercycler X50 PCR cycler heats at 10 °C/s and cools at 5 °C/s, so make the speed of the MC X50 your research advantage.
Speed is more than just the ramp rate
There is a wide range of PCR thermocyclers available, with differing claims about ramp rates. However, if you compare the same protocol using different PCR cyclers, you see that ramp rates don’t tell the full story about run time speed. At Eppendorf, we don’t just make claims, we back them up; so we’ve done the performance comparison which shows that the Mastercycler X50 really does give you reliable ramp rates and real PCR speed. To see the full report, see Application Note 274.
Improving ergonomics across the lab
Ergonomics goes far beyond just the »ergonomically designed chair«. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.
In 2003, we launched the PhysioCare Concept®, focusing on ergonomic liquid handling devices, like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept has broadened to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and well-being.
For the Eppendorf Mastercyler range, this means:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling
Simple, intuitive documentation and tracking
The eLABJournal® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.