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Eppendorf Mastercycler® X50

Available as standalone units, controlled by the user-friendly VisioNize^® touchscreen interface, or externally controlled eco units, the Mastercycler X50 family offers the flexibility to meet all your throughput needs.Each standalone PCR thermocycler can be networked with up to nine additional eco units. For even higher throughput, you can control up to fifty MC X50 eco units with our CycleManager X50 software. Whichever you need, you’ll be able to monitor and manage every unit – individually, simultaneously or as a group – with the assurance that every PCR cycler provides excellent block homogeneity, accuracy and precision. For security, simply choose different levels of access for different users. Low noise and power usage, and the versatile flexlid^® concept for maximized consumable flexibilty, make the MC X50 the most powerful and convenient PCR thermocycler set-up.

Advanced PCR Optimization in a One Step

Mastercycler X50 PCR thermocyclers have a 2D-gradient function that means you can simultaneously optimize both the denaturation temperature (bottom to top) and the annealing temperature (left to right) – see the example of β-actin gene PCR optimization.

Higher denaturation temperatures can increase specificity while lower denaturation temperatures can increase yield, so with the MC X50 PCR cyclers, you can quickly determine the conditions for both optimal yield and specificity. This saves PCR optimization time, is a great benefit in low target copy number and GC-rich target applications, and is really useful in troubleshooting non-specific amplification issues.

Need rapid PCR results?

In the modern lab, fast is essential, not a luxury. PCR thermocyclers that give you quicker results throughout the day and a faster transition to optimal temperatures mean you can get through more work in a shorter time. The Mastercycler X50 PCR cycler heats at 10 °C/s and cools at 5 °C/s, so make the speed of the MC X50 your research advantage.

Speed is more than just the ramp rate

There is a wide range of PCR thermocyclers available, with differing claims about ramp rates. However, if you compare the same protocol using different PCR cyclers, you see that ramp rates don’t tell the full story about run time speed. At Eppendorf, we don’t just make claims, we back them up; so we’ve done the performance comparison which shows that the Mastercycler X50 really does give you reliable ramp rates and real PCR speed. To see the full report, see Application Note 274.

Improving ergonomics across the lab

Ergonomics goes far beyond just the »ergonomically designed chair«. At Eppendorf, we began optimizing our laboratory devices for ergonomics in the early 1970s.

In 2003, we launched the PhysioCare Concept^®, focusing on ergonomic liquid handling devices, like our pipettes.

Nowadays, the Eppendorf PhysioCare Concept has broadened to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and well-being.

For the Eppendorf Mastercyler range, this means:
› Ergonomically designed lid closure
› Low noise level for convenient working conditions at the bench
› Small footprint to enable position in direct arm reach at your bench
› Standardized »user-interface concept« for intuitive handling of devices
› Guided software for easy handling

Simple, intuitive documentation and tracking

The eLABJournal^® Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data, including optimal PCR cycler temperatures and results. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.

Eppendorf Mastercycler® X50

	MC X50s	MC X50a	MC X50p	MC X50h	MC X50i	MC X50l	MC X50r	MC X50t
Touchscreen Interface	￭	￭	￭	￭	–	–	–	–
Unit can control 9 other units in network	￭	￭	￭	￭	–	–	–	–
Unit can only be controlled	–	–	–	–	￭	￭	￭	￭
Thermoblock	Silver	Aluminum	Aluminum	Aluminum	Silver	Aluminum	Aluminum	Aluminum
Sample capacity	96-well plate or 0.1/0.2 mL tubes	96-well plate or 0.1/0.2 mL tubes	1 PCR plate 96	1 PCR plate 384	96-well plate or 0.1/0.2 mL tubes	96-well plate or 0.1/0.2 mL tubes	1 PCR plate 96	1 PCR plate 384
96-well plate	￭	￭	￭	–	￭	￭	￭	–
384-well plate	–	–	–	￭	–	–	–	￭
0.1/0.2 mL tubes	￭	￭	–	–	￭	￭	–	–
Heating technology	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements	Six peltier elements
2D-Gradient block	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 24 columns/over 16 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 12 columns/over 8 rows	over 24 columns/over 16 rows
Temperature control mode	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe	Fast, Intermediate, Standard, Safe
Temperature control range of the block	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C	4 – 99 °C
Gradient range	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C	1 – 30 °C
Gradient temperature control range	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C	30 – 99 °C
Block homogeneity 20 – 72 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C	≤±0.2 °C
Block homogeneity 95 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C	≤±0.3 °C
Control accuracy	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C	±0.15 °C
Cooling rate	5 °C/s	2.3 °C/s	2.3 °C/s	2.3 °C/s	5 °C/s	2.3 °C/s	2.3 °C/s	2.3 °C/s
Lid descent	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP	flexlid^® technology with TSP
High pressure lid	–	–	￭	￭	–	–	￭	￭
Lid temperature range	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C	37 – 110 °C
Interfaces	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB	Ethernet, USB
Power supply	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz	110 – 230 V, 50 – 60 Hz
Max. power consumption	850 W	850 W	850 W	850 W	850 W	850 W	850 W	850 W
Total weight	11.5 kg	11.5 kg	11.5 kg	11.5 kg	10.7 kg	10.7 kg	10.7 kg	10.7 kg
Dimensions (W × D × H)	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in	27.5 × 43 × 33 cm / 10.8 × 16.9 × 13 in
Eppendorf ThermoMixer^®
Heating rate	10 °C/s	5 °C/s	5 °C/s	5 °C/s	10 °C/s	5 °C/s	5 °C/s	5 °C/s

Brochure – Master of Class

Brochure – Simple, Central, Smart

Brochure – The Next Stage

Customer Testimonial – Interview Henner Tasch

Eppendorf produktkatalog

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